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Human anti-HCV(anti-Hepatitis C antibody) ELISA Kit
ELK0923
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48 T $320.00
96 T $458.00
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Overview

Product name: Human anti-HCV(anti-Hepatitis C antibody) ELISA Kit
Reactivity: Human
Alternative Names: Hepatitis C virus IgG
Assay Type: Sandwich
Sensitivity: 0.12 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human anti-HCV. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human anti-HCV. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human anti-HCV, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human anti-HCV in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.056 1.972
10.00 1.527 1.443
5.00 1.253 1.169
2.50 0.819 0.735
1.25 0.509 0.425
0.63 0.335 0.251
0.32 0.170 0.086
0.00 0.084 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant anti-HCV and the recovery rates were calculated by comparing the measured value to the expected amount of anti-HCV in samples.
Matrix Recovery range Average
serum(n=5) 91-105% 98%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 82-98% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of anti-HCV and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-99% 79-93% 81-96% 85-96%
EDTA plasma(n=5) 87-105% 83-92% 82-96% 88-101%
Heparin plasma(n=5) 95-106% 85-104% 90-98% 89-105%