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Human CK 18-M30 (Cytokeratin 18-M30) ELISA Kit
ELK0871
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48 T $320.00
96 T $458.00
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Overview

Product name: Human CK 18-M30 (Cytokeratin 18-M30) ELISA Kit
Reactivity: Human
Alternative Names: K18-M30; CK-18-M30; CYK18-M30
Assay Type: Sandwich
Sensitivity: 7.1 μIU/mL
Standard: 1000 μIU/mL
Detection Range: 15.63-1000 μIU/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cancer of the liver; Viral hepatitis
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CK 18-M30. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CK 18-M30. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CK 18-M30, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CK 18-M30 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (μIU/mL) OD Corrected OD
1000.00 1.972 1.883
500.00 1.507 1.418
250.00 1.107 1.018
125.00 0.671 0.582
62.50 0.479 0.390
31.25 0.312 0.223
15.63 0.196 0.107
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CK 18-M30 and the recovery rates were calculated by comparing the measured value to the expected amount of CK 18-M30 in samples.
Matrix Recovery range Average
serum(n=5) 85-95% 90%
EDTA plasma(n=5) 88-103% 95%
Heparin plasma(n=5) 83-95% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CK 18-M30 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 91-103% 86-92% 89-97%
EDTA plasma(n=5) 88-104% 87-96% 85-93% 82-90%
Heparin plasma(n=5) 95-102% 89-96% 87-98% 85-93%