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Human Acetyl-H3(Acetyl Histone H3) ELISA Kit
ELK0808
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48 T $265.00
96 T $376.00
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Overview

Product name: Human Acetyl-H3(Acetyl Histone H3) ELISA Kit
Reactivity: Human
Alternative Names: H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; ; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l;
Assay Type: Sandwich
Sensitivity: 0.16 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Epigenetics and Nuclear Signaling
Uniprot ID: P68431
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Acetyl-H3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Acetyl-H3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Acetyl-H3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Acetyl-H3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 1.929 1.831
10.00 1.673 1.575
5.00 1.223 1.125
2.50 0.806 0.708
1.25 0.541 0.443
0.63 0.369 0.271
0.32 0.179 0.081
0.00 0.098 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Acetyl-H3 and the recovery rates were calculated by comparing the measured value to the expected amount of Acetyl-H3 in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 90-103% 96%
Heparin plasma(n=5) 93-107% 100%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acetyl-H3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 95-102% 89-101% 95-104%
EDTA plasma(n=5) 93-102% 88-102% 95-102% 81-93%
Heparin plasma(n=5) 85-95% 82-91% 86-93% 81-94%