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Human Anti-tTG-IgA (Anti-Tissue Transglutaminase IgA) ELISA Kit
ELK0752
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human Anti-tTG-IgA (Anti-Tissue Transglutaminase IgA) ELISA Kit
Reactivity: Human
Alternative Names: Anti-Tissue Transglutaminase IgA
Assay Type: Sandwich
Sensitivity: 1.47 IU/mL
Standard: 300 IU/mL
Detection Range: 4.69-300 IU/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Endocrinology;Neuro science;Autoimmunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG-IgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG-IgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG-IgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-tTG-IgA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (IU/mL) OD Corrected OD
300.00 2.075 1.988
150.00 1.472 1.385
75.00 1.069 0.982
37.50 0.728 0.641
18.75 0.535 0.448
9.38 0.303 0.216
4.69 0.191 0.104
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-tTG-IgA and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-tTG-IgA in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 82-97% 89%
Heparin plasma(n=5) 87-95% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-tTG-IgA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-102% 83-96% 97-105% 85-97%
EDTA plasma(n=5) 78-91% 86-92% 89-96% 91-97%
Heparin plasma(n=5) 81-93% 78-91% 87-98% 95-107%