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Human Anti-tTG IgG (Anti-Tissue Transglutaminase IgG) ELISA Kit
ELK0751
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48 T $320.00
96 T $458.00
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Overview

Product name: Human Anti-tTG IgG (Anti-Tissue Transglutaminase IgG) ELISA Kit
Reactivity: Human
Alternative Names: Anti-Tissue Transglutaminase IgG
Assay Type: Sandwich
Sensitivity: 0.59 ng/mL
Standard: 100 ng/mL
Detection Range: 1.56-100 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Endocrinology;Neuro science;Autoimmunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-tTG IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.035 1.940
50.00 1.663 1.568
25.00 1.121 1.026
12.50 0.815 0.720
6.25 0.585 0.490
3.13 0.549 0.454
1.57 0.203 0.108
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-tTG IgG and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-tTG IgG in samples.
Matrix Recovery range Average
serum(n=5) 82-94% 88%
EDTA plasma(n=5) 85-97% 91%
Heparin plasma(n=5) 83-95% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-tTG IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-97% 82-96% 86-98% 93-101%
EDTA plasma(n=5) 87-103% 79-92% 86-99% 84-97%
Heparin plasma(n=5) 88-97% 95-106% 83-98% 93-105%