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Human Anti-RyR(Anti-ryanodine receptor calcium release channel antibody) ELISA Kit
ELK0746
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48 T $320.00
96 T $458.00
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Overview

Product name: Human Anti-RyR(Anti-ryanodine receptor calcium release channel antibody) ELISA Kit
Reactivity: Human
Alternative Names: Anti-ryanodine receptor antibody
Assay Type: Competitive Inhibition
Sensitivity: 0.24 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Signal Transduction; Neuroscience
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-RyR protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-RyR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-RyR in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
50.00 0.217
25.00 0.467
12.50 0.722
6.25 0.997
3.13 1.297
1.57 1.557
0.79 1.869
0.00 2.256

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-RyR and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-RyR in samples.
Matrix Recovery range Average
serum(n=5) 83-99% 95%
EDTA plasma(n=5) 85-97% 91%
Heparin plasma(n=5) 85-97% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-RyR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 93-103% 94-106% 91-101%
EDTA plasma(n=5) 88-101% 93-106% 85-95% 92-104%
Heparin plasma(n=5) 90-99% 83-97% 81-103% 89-101%