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Human Anti-GM1(Anti-Ganglioside Antibody) ELISA Kit
ELK0676
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48 T $320.00
96 T $458.00
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Overview

Product name: Human Anti-GM1(Anti-Ganglioside Antibody) ELISA Kit
Reactivity: Human
Alternative Names: anti-ganglioside (GM1) antibody (IgG)
Assay Type: Sandwich
Sensitivity: 0.57 ng/mL
Standard: 80 ng/mL
Detection Range: 1.25-80 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-GM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-GM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-GM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
80.00 2.153 2.051
40.00 1.755 1.653
20.00 1.223 1.121
10.00 0.933 0.831
5.00 0.553 0.451
2.50 0.372 0.270
1.25 0.251 0.149
0.00 0.102 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-GM1 and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-GM1 in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 80-97% 88%
Heparin plasma(n=5) 78-92% 85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-GM1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-99% 86-92% 89-103% 97-105%
EDTA plasma(n=5) 95-104% 87-96% 82-96% 89-103%
Heparin plasma(n=5) 85-92% 89-96% 90-98% 87-98%