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Simian C5b-9(Terminal Complement Complex C5b-9) ELISA Kit
ELK0538
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48 T $320.00
96 T $458.00
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Overview

Product name: Simian C5b-9(Terminal Complement Complex C5b-9) ELISA Kit
Reactivity: Simian
Alternative Names: MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9
Assay Type: Sandwich
Sensitivity: 0.14 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Metabolic pathway;Apoptosis;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian C5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian C5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian C5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian C5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.179 2.086
10.00 1.625 1.532
5.00 1.109 1.016
2.50 0.897 0.804
1.25 0.655 0.562
0.63 0.365 0.272
0.32 0.219 0.126
0.00 0.093 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant C5b-9 and the recovery rates were calculated by comparing the measured value to the expected amount of C5b-9 in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 80-94% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C5b-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-95% 82-93% 97-106% 89-102%
EDTA plasma(n=5) 86-95% 93-103% 87-98% 85-96%
Heparin plasma(n=5) 86-94% 91-105% 87-99% 95-100%