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Cattle β2-MG(Beta-2-Microglobulin) ELISA Kit
ELK0528
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48 T $265.00
96 T $376.00
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Overview

Product name: Cattle β2-MG(Beta-2-Microglobulin) ELISA Kit
Reactivity: Cattle
Alternative Names: Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin
Assay Type: Sandwich
Sensitivity: 0.61 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma, urine and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;Kidney biomarker;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle β2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle β2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle β2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle β2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.291 2.202
50.00 1.562 1.473
25.00 1.186 1.097
12.50 0.975 0.886
6.25 0.572 0.483
3.13 0.368 0.279
1.57 0.226 0.137
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant β2-MG and the recovery rates were calculated by comparing the measured value to the expected amount of β2-MG in samples.
Matrix Recovery range Average
serum(n=5) 78-92% 85%
EDTA plasma(n=5) 78-90% 84%
Heparin plasma(n=5) 85-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of β2-MG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-97% 93-105% 82-93% 95-104%
EDTA plasma(n=5) 79-95% 96-105% 85-96% 90-99%
Heparin plasma(n=5) 88-97% 95-106% 83-98% 93-105%