E1(Estrone) ELISA Kit
Specification: Price:
48 T $265.00
96 T $376.00


Product name: E1(Estrone) ELISA Kit
Reactivity: General
Alternative Names: Oestrone
Assay Type: Competitive Inhibition
Sensitivity: 8.86 pg/mL
Standard: 2000 pg/mL
Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Metabolic pathway;Endocrinology;Reproductive science;Genetic science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Estrone(E1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Estrone(E1). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrone(E1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
2000.00 0.189
1000.00 0.367
500.00 0.621
250.00 0.833
125.00 1.197
62.50 1.522
31.25 1.775
0.00 2.123


Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.


Matrices listed below were spiked with certain level of recombinant E1 and the recovery rates were calculated by comparing the measured value to the expected amount of E1 in samples.
Matrix Recovery range Average
serum(n=5) 85-99% 92%
EDTA plasma(n=5) 93-107% 100%
Heparin plasma(n=5) 87-99% 97%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-98% 83-103% 89-102% 92-101%
EDTA plasma(n=5) 79-93% 89-97% 85-94% 87-101%
Heparin plasma(n=5) 91-99% 87-96% 96-105% 95-103%