(1) Centrifuge dry primer powder at 13,000 rpm for 1 min at room temperature before opening the lid. (2) Add DEPC water with 10 times nmole value of water (μL) on the tube wall, vortex to mix, and centrifuge briefly. This is the primer stock solution (100 μM), which is stored at -20°C after
Because of its easy operation,enzyme-linked immunosorbent assay (ELISA) can simply answer the basic question of how many proteins/polypeptides/antibodies are in the sample. It is a commonly used detection method in scientific research.The core of this simple analysis method is the standard curve.Wit